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投稿时间：2022-04-25
投稿时间：2022-04-25
中文摘要: 以新鲜黄牛血为原料，利用离心、裂解等方法提取抗菌肽，在单因素试验的基础上通过正交试验研究牛血离心转速、NH4Cl 溶液与样品体积比、超声波功率和超声时间对粗抗菌肽得率的影响。结果表明：最佳提取条件为牛血离心转速3 500 r/min、NH4Cl 溶液与样品体积比3 ∶1、超声功率600 W、超声时间8 min，验证得粗抗菌肽得率可达0.008 5%；进一步采用聚丙烯酰胺凝胶电泳法确定抗菌肽的分子量，并通过电洗脱分离纯化的抗菌肽，结果表明抗菌肽的分子量在14 kDa 左右，电洗脱回收率为82%。
Abstract:Antimicrobial peptides were extracted by centrifugation and lysis with fresh cow blood as raw material.Following single-factor test，the effects of centrifuge speed，ratio of NH4Cl solution to sample volume，ultrasonic power and ultrasonic time on the yield of crude antimicrobial peptides were investigated by orthogonal test.The results revealed that the optimal extraction conditions were bovine blood centrifuge speed 3 500 r/min，ratio of NH4Cl solution to sample volume 3 ∶1，ultrasonic power 600 W and ultrasonic time 8 min，which were verified to promote the yield of antibacterial peptides up to 0.008 5%. Further, the molecular weight of the antimicrobial peptide was determined by polyacrylamide gel electrophoresis，and the purified antimicrobial peptide was separated by electroelution. It was found that the molecular weight of the antimicrobial peptides was around 14 kDa，and the recovery by electroelution was 82%.
keywords: fresh cow blood antimicrobial peptide polyacrylamide gel electrophoresis electroelution aczoiling
文章编号：202313022 中图分类号： 文献标志码：
基金项目:大学生国家级科技创新课题（202111439022）；吉林省教育厅科学技术研究项目（JJKH20210405KJ）
| Author Name | Affiliation |
| WANG Yabei，LIU Yabin，DING Lanhui，DENG Haoren，CHENG Jiajia，YANG Ping* | School of Food Engineering，Jilin Agricultural Science and Technology University，Jilin 132101，Jilin，China |
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