Abstract:This study aims to explore the conditions for extraction of target components（polysaccharides，total flavonoids，and total saponins）from the fruit pulp of Rosa davurica Pall.by circulating column chromatography and the in vitro activity of the components.With the content of the target components in the extraction solution as the evaluation index，such as ethanol volume fraction，swelling volume，soaking equilibrium time，and ultrasonic time were investigated to determine the optimal process. The α-glucosidase-inhibiting rate and the nitritescavenging rate of target components in extracts of the fruit pulp R.davurica were measured.The results showed that under the optimum extraction conditions，the extraction rates of three target components in the fruit pulp of R.davurica were more than 90%.The content of polysaccharides，total flavonoids，and total saponins in the extract was 428.5，484.5 mg/g，and 122.5 mg/g，respectively. After purification，the content of polysaccharides，total flavonoids，and total saponins in the dry extracts was 804.1，772.7 mg/g，and 396.4 mg/g，respectively.The in vitro α-glucosidase-inhibiting activity of target components was stronger than that of acarbose，suggesting the significant in-vitro hypoglycemic effect of the components. The IC50 value of the extracts of the target components on nitrite was higher than that of the positive control vitamin C，indicating the weak nitrite-scavenging activity. Column chromatography can simultaneously extract polysaccharides，total flavonoids，and total saponins with high efficiency.This study lays a basis for the development of the fruit products of R.davurica.