Abstract:Inhibition of pancreatic lipase activity can effectively reduce the incidence of obesity.In this study，the inhibitory effect of naringenin on pancreatic lipase was studied through enzymatic kinetics，fluorescence quenching，and molecular docking.The enzyme activity experiment showed that naringenin inhibited pancreatic lipase activity in a reversible competitive manner，with the half inhibitory concentration of 2.413 mmol/L.The fluorescence quenching experiment showed that the binding with naringenin quenched the fluorescence and changed the conformation of pancreatic lipase.Molecular docking results showed that naringenin and pancreatic lipase formed an enzyme-inhibitor complex，which prevented the binding of enzyme with substrate，decreasing the enzyme catalytic activity.