Abstract:Acroporous resin was employedto purify Eleocharis tuberosa peel polyphenols，and their antioxidant activity and stability were studied.The results showed that AB-8 resin was the most suitablefor the purification of E.tuberosa peel polyphenols among AB-8，S-8，NKA-9，D101 and ADS-17 resins，and the static adsorption basically reached saturation after 140 min，with the adsorption capacity of（31.25±1.09）mg/g.The optimal elution conditions were the initial concentration of polyphenols 3.0 mg/mL，flow rate 4 mL/min，using 70% ethanol as eluent after adsorption equilibrium and elution flow rate 2 mL/min.After macroporous resin adsorption and purification，the purity of E.tuberosa peel polyphenols increased from 12.54% to（58.64±1.76）%.By determining and comparing the scavenging rates of 2，2'-azinobis-（3-ethylbenzthiazoline-6-sulphonate）cation（ABTS+）and 1，1-diphenyl-2-picrylhydrazyl（DPPH） free radicals before and after purification，the authors found that the purified polyphenols had high antioxidant activity.The results of temperature，pH，light and sucrose influencing the stability of E.tuberosa peel polyphenols demonstrated that the thermal stability of polyphenols was poor，but they were stable under the conditions of pH3，light and sucrose.