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投稿时间：2021-10-07
投稿时间：2021-10-07
中文摘要: 为获得可高产β-苯乙醇的菌株，以β-苯乙醇作为筛选压力进行酵母菌筛选。成功获得一株可耐受4 g/L β-苯乙醇浓度的酵母菌YDF-1，可产2.83 g/L β-苯乙醇。通过形态学特征和18S rDNA分析鉴定，YDF-1为库德里阿兹威毕赤酵母。并通过单因素和响应面试验优化YDF-1增殖条件，最终优化培养基为葡萄糖100 g/L、胰蛋白胨3 g/L、KH2PO44 g/L、MgSO4·5H2O 0.5 g/L、CuSO40.25 g/L、MnCl20.142 g/L。在以接种量4% ，转速250 r/min，30℃的条件下培养20 h，YDF-1可增殖到1.39×109CFU/mL，相比未优化培养基增加了2.6倍以上。并以此为基础进行酵母增殖转化生产β-苯乙醇，48 h发酵液中β-苯乙醇含量可达到3.27 g/L。
中文关键词: β-苯乙醇 库德里阿兹威毕赤酵母 18S rDNA 响应面试验 优化培养基
Abstract:To obtain yeast strains that produce high β-phenylethanol yields，β-phenylethanol was used as the screening pressure.The results showed that the YDF-1 strain tolerating 4 g/L of β-phenylethanol was successfully screened and produced 2.82 g/L of β-phenylethanol.Based on morphological characteristics and 18S rDNA sequence analysis，YDF-1 was identified as Pichia kudriavzevii.The optimal medium contained 100 g/L glucose，3 g/L tryptone，4 g/L KH2PO4，0.5 g/L MgSO4·5H2O，0.25 g/L CuSO4，and 0.142 g/L MnCl2.The optimized liquid medium was inoculated at 4% and cultured at 30 ℃ for 20 h at 250 r/min；the bacterial concentration was 1.39×109CFU/mL，which was more than 2.6 times that of the non optimized medium.This study provides the experimental basis and technical reference to obtain β-phenylethanol through fermentation.High-density conversion was conducted to produce β-phenylethanol，and its concentration in 48 h fermentation medium reached 3.27 g/L.
文章编号：202216022 中图分类号： 文献标志码：
基金项目:
| 作者 | 单位 |
| 刘子睦1，李娜1，项馨瑶1，薛鲜丽1，2，王德培1，3* | 1.天津科技大学生物工程学院，天津 300457；2.工业发酵微生物教育部重点实验室，天津 300457；3.天津市微生物代谢与发酵过程控制技术工程中心，天津 300457 |
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