Abstract:In order to obtain a lactic acid bacteria strain with high safety，high efficiency，and strong resistance，the growth characteristics and drug resistance spectrum of Enterococcus faecium RS3 isolated from kimchi were systematically evaluated.The results showed that E.faecium RS3 is a Gram-positive，facultative anaerobic bacteria，and cells are elliptical or round，distributed in clusters or short chains of 4-5 cells.Tetradecanoic acid，hexadecanoic acid，hexadecenoic acid，octadecenoic acid and octadecadienoic acid were the characteristic peaks of fatty acids in E.faecium RS3 cells.The strain could ferment sorbitol and D-（+）-raffinose，but could not ferment L-arabinore and mannitol，and produced 5.73 g/L lactic acid in the skimmed milk medium.The strain was sensitive to ampicillin but resistant to cephalothiophene and other indicated antibiotics.These data provide a theoretical reference for further evaluation and development of probiotics by using E.faecium RS3.