Abstract:Functional sugar has special effects such as low calorie，improving intestinal flora，preventing dental caries，and lowering blood sugar，whose consumption accounting for about 24% .Therefore，development of green manufacturing technology for functional sugars is a major demand for people's life and health and the development of the national economy.To solve the problems of difficult recycling and poor stability of enzymes or cells in the biosynthesis of functional sugars，many immobilization technologies had been developed for the biomanufacturing of functional sugars.This review systematically introduced the immobilized enzymes and immobilized cells for the synthesis of functional sugars，which aimed to provide guidance for the development and promotion of key technologies for green biomanufacturing.