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投稿时间:2022-03-31
投稿时间:2022-03-31
中文摘要: 以蛹虫草菌种为材料,高粱、糜子、油莎豆3 种谷物为培养基质,测定谷物蛹虫草菌丝共生体和蛹虫草子实体的主要活性成分,研究不同培养基质对蛹虫草有效成分含量的影响。结果表明,油莎豆培养的蛹虫草菌丝共生体产纤溶酶酶活最高,为312.26 U/g;油莎豆培养的蛹虫草子实体产虫草素含量最高,为7.34 mg/g。高粱培养的蛹虫草子实体麦角甾醇含量最高,为6.10 mg/g;糜子蛹虫草菌丝共生体粗多糖含量最高,为72.5 mg/g。高粱适合作为培养高产麦角甾醇的蛹虫草子实体的培养基质,糜子适合作为培养高产粗多糖的菌丝共生体的培养基质,油莎豆适合作为培养高产虫草素的蛹虫草子实体和高产纤溶酶的菌丝共生体的培养基质。
Abstract:This study aims to investigate the effect of medium on the active ingredient content of Cordyceps militaris.To be specific,the active ingredient content in the sorghum-C.militaris mycelium,millet-C.militaris mycelium,and tiger nut-C. militaris mycelium symbiotes and fruiting bodies was determined. The results showed that the activity of fibrinolytic enzyme of the tiger nut-C. militaris mycelium symbiote was the highest,up to 312.26 U/g. The cordycepin content of the fruiting bodies cultured on the tiger nut was the highest,reaching 7.34 mg/g.The ergosterol content in C.militaris fruiting bodies on the sorghum medium and the crude polysaccharide content in millet-C. militaris mycelium symbiote was the maximum,which was 6.10 mg/g and 72.5 mg/g,respectively.Sorghum is the optimum for culturing C.militaris with high ergosterol content in fruiting bodies,and millet is optimal for culturing C.militaris with high content of crude polysaccharide.Moreover,tiger nut is suitable for culturing C. militaris with high cordycepin content in the fruiting bodies and high yield of fibrinolytic enzyme mycelium,respectively.
keywords: Cordyceps militaris grain mediums crude polysaccharide cordycepin ergosterol fibrinolyti enzyme
文章编号:202215004 中图分类号: 文献标志码:
基金项目:2020 年度黑龙江省省属高等学校基本科研业务费科研项目(135509324);黑龙江省玉米深加工理论与技术重点实验室开放课题项目(SPKF202013)
作者 | 单位 |
钱朋智1,张梅娟2*,王韬2,郭宏文1,王艳菊1,甄珍3 | 1.齐齐哈尔大学食品与生物工程学院,黑龙江 齐齐哈尔 161006;2.齐齐哈尔大学生命科学与农林学院,黑龙江 齐齐哈尔 161006;3.黑龙江省哈尔滨市海关技术中心齐齐哈尔综合实验室,黑龙江 齐齐哈尔 161000 |
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