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食品研究与开发:2022,43(10):62-69
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低压静电场对采后马铃薯块茎愈伤效果的影响
(1.天津商业大学生物技术与食品科学学院天津市食品生物技术重点实验室,天津 300134;2.中国农业科学院农产品加工研究所,北京 100193)
Effects of Low-Voltage Electrostatic Field Treatment on Wound Healing in Postharvest Potato Tubers
(1.Tianjin Key Laboratory of Food Biotechnology,College of Biotechnology and Food Science,Tianjin University of Commerce,Tianjin 300134,China;2.Institute of Agro-products Processing Science and Technology,Chinese Academy of Agricultural Sciences,Beijing 100193,China)
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投稿时间:2021-09-15    
中文摘要: 为研究低压静电场处理对采后马铃薯块茎愈伤效果的影响,以“冀张薯12号”马铃薯块茎为试材,人工模拟机械损伤后,置于有低压静电场的常温[(20±2)℃]、相对湿度85%~90%、黑暗条件下愈伤,通过测定块茎愈伤期间的失重率、损伤伤口处硬度、褐变度、苯丙烷代谢关键酶活性和代谢产物含量的变化,以及分析低压静电场对过氧化物酶、多酚氧化酶活性和H2O2含量的影响来评价愈伤效果。结果表明,在愈伤期间低压静电场处理能够有效减少损伤块茎的重量损失,显著促进损伤部位褐变度值和硬度的增加,显著提高伤口处组织中苯丙氨酸解氨酶活性,促使总酚、类黄酮和木质素含量增加,此外,低压静电场处理增加了块茎伤口处的多酚氧化酶、过氧化物酶活性和H2O2含量,结果显示,低压静电场处理能够促进采后马铃薯块茎的愈伤。
中文关键词: 低压静电场  马铃薯  块茎  损伤  愈伤
Abstract:The effects of low-voltage electrostatic field treatment on the healing effect of post-harvest potato tubers were investigated in'Jizhangshu No.12'potato tubers,which served as the test material.After artificial mechanical damage,the calluses were placed under normal temperature(20±2)℃ and relative humidity(85%-90%)conditions in the dark and subjected to a low-voltage electrostatic field.Changes in weight-loss rate,hardness of injury wounds,browning degree,activity of key enzymes associated with phenylpropane metabolism,and contents of metabolites were measured.The effects of the low-voltage electrostatic field on peroxidase,polyphenol oxidase activity,and hydrogen peroxide (H2O2)contents were analyzed to evaluate the effects of the electrostatic field on the calluses.During the callus period,the low-voltage electrostatic field treatment effectively reduced the weight loss of damaged tubers and significantly promoted increase of the browning degree and hardness of the damaged parts.After the low-voltage electrostatic field treatment,the activity of phenylalanine ammonia lyase in the tuber wounds increased significantly and the contents of total phenols,flavonoids,and lignin also increased.In addition,the low-voltage electrostatic field treatment also increased the activities of polyphenol oxidase and peroxidase and H2O2contents in the tuber wounds.These results showed that low-voltage electrostatic field treatment can promote callus formation in postharvest potato tubers.
文章编号:202210009     中图分类号:    文献标志码:
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