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投稿时间：2021-10-12
投稿时间：2021-10-12
中文摘要: 对实验室已经进行16S rDNA鉴定的来源于浓香型白酒窖泥的近似梭菌进行随机扩增多态性DNA标记（random amplified polymorphic DNA，RAPD）分型，19株梭菌基于16S rDNA序列分析可以分为3类，分别与拜氏梭菌（Clostridium beijerinckii）、丁酸梭菌（Clostridium butyricum）和广西梭菌（Clostridium guangxiense）近似。结果表明，通过筛选获得4个具有良好的分型效果的随机引物，基于其RAPD扩增图谱的0/1矩阵分析可将这19株梭菌分成10类。其中与拜氏梭菌类菌群近似的11株菌株分为6类，包括2-1类、4-30类、C2-2类、G3-1类、G3-5类和JL3-1类；将与丁酸梭菌类菌群近似的6株菌分为2类，包括3-3类和JL3-4类；将广西梭菌类菌群近似的2株菌分为2类包括B2-1类、F1-1类。
中文关键词: 浓香型白酒 窖泥 梭菌近似菌株 随机扩增多态性DNA标记 分类
Abstract:Randomly amplified polymorphic DNA （RAPD）was used to distinguish between similar strains of Clostridium isolated from the pit mud of strong-flavor Baijiu fermentation cellars，based on 16S rDNA sequences.Nineteen similar Clostridium strains were divided into the following three categories based on the analysis:those similar to Clostridium beijerinckii，C.butyricum，or C.guangxiense.Four random primers with good distinguishing capabilities were identified by screening experiments.Based on the 0/1 matrix analysis of RAPD amplification electrophoretograms，the 19 Clostridium strains were grouped into 10 categories.Eleven of these strains were similar to C.beijerinckii and were divided further into the following six categories:2-1，4-30，C2-2，G3-1，G3-5，and JL3-1.Six strains were similar to C.butyricum and divided into two categories，3-3 and JL3-4，while two strains similar to C.guangxiense were divided into the categories B2-1 and F1-1.
keywords: strong-flavor Baijiu pit mud Clostridium similar strains random amplified polymorphic DNA（RAPD） taxonomy
文章编号：202206023 中图分类号： 文献标志码：
基金项目:2018年四川理工学院校级人才引进项目（2018RCL27）；2016年四川理工学院院士专家工作站项目（GY2016-02）；2021年四川轻化工大学创新创业项目（cx2121137）
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