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投稿时间：2021-11-08
投稿时间：2021-11-08
中文摘要: 为实现漆酶基因稳定高效的异源表达，利用基因工程技术构建同源整合表达载体pGQLR，删除其来自原核的DNA序列后电转化至产朊假丝酵母菌（Candida utilis，C.utilis），构建一株表达漆酶的产朊假丝酵母工程菌ZHQX1，并对其遗传稳定性和酶活进行评价。ZHQX1在连续传代100代时外源基因仍稳定存在，保持了良好的遗传稳定性，培养96h时其漆酶酶活是同时期原始菌—云芝栓孔菌酶活的4.8倍，达到1078.9U/L。
Abstract:In order to achieve stable and efficient heterologous expression of laccase gene，the homologous expression vector pGQLR was constructed by genetic engineering and then deleted the prokaryotic DNA sequence of pGQLR.The resultant product was finally transferred into C.utilis.A genetically engineered strain of Candida utilis-ZHQX1 was developed and detected the genetic stability and enzyme activity.ZHQX1 maintained high genetic stability，as the foreign gene remained stable in ZHQX1 after 100 generations.The laccase activity of ZHQX1 was 1 078.9 U/L，4.8 times higher than that of Trametes versicolor after 96 h of incubation.
文章编号：202206022 中图分类号： 文献标志码：
基金项目:内蒙古自治区科技计划项目（2019GG354）
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