Abstract:This study aimed to evaluate the substantial equivalence between glyphosate-resistant genetically modified soybeans and non-transgenic soybeans.The content of conventional nutrients，amino acids，fatty acids，and some anti-nutritional factors was determined in these two varieties.There was little difference in the crude protein content between the two soybean varieties.The content of 17 amino acids（except tyrosine）was lower in genetically modified soybeans than in non-transgenic soybeans.The content of aspartic acid，glutamic acid，and arginine was much lower in genetically modified soybeans than in non-transgenic soybeans.The crude fat content was higher in genetically modified soybeans than in non-transgenic soybeans.Moreover，there were certain differences in the types and content of fatty acids between the two varieties.In total，13 and 8 types of fatty acids were detected in genetically modified soybeans and non-transgenic soybeans，respectively.The content of palmitic acid，stearic acid，and oleic acid was much higher in genetically modified soybeans than in non-trans－genic soybeans.Glycinin content was higher while trypsin inhibitor content was lower in genetically modified soybeans than in non-transgenic soybeans.In conclusion，glyphosate-resistant genetically modified soybeans and non-transgenic soybeans are not substantially equivalent in nutrition.