Abstract:The application of Saccharomyces cerevisiae with high yield of ester and low yield of higher alcohol in the production of distiller's grains production was studied with the aim of effectively reducing grain consumption in the liquor industry and improving the quality of distiller's grains.The optimal technological conditions were determined by varying the ratio of saccharifying agent，S.accharomyces cerevisiae species and amount of yeast inoculum.The optimum technological conditions were bran koji UV-11，S.cerevisiae PY-1 and 600 million yeast/100 g distiller's grains，respectively.Under these conditions，the alcohol content in fermented grains reached 3.4% vol and the residual starch decreased to 6.4%，resulting in greater utilization of the starch in spent grains.Compared with the control group，the content of main esters was greatly increased.Ethyl acetate，ethyl lactate and total esters increased by 21 times，8.8% and 28.0%，respectively.The main higher alcohols were reduced，including phenethyl alcohol by 44% and total higher alcohols by approximately 38.5%.