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投稿时间：2021-01-08
投稿时间：2021-01-08
中文摘要: 根据对大肠杆菌、金黄色葡萄球菌、白色念珠菌的抑菌能力及广谱性，从9种植物提取物（以下简称植提物）中筛选出4种，再通过棋盘稀释法复配得到一种植物源防腐剂，以大肠杆菌为供试菌研究其抑菌机理。结果表明，筛选出的4种植提物为连翘（Forsythia suspensa）精油、无花果（Ficus carica Linn）精油、丁香（Syzygium aromaticum）精油、紫苏（Perilla frutescens）提取物；通过棋盘稀释法得到的植物源防腐剂最佳配方为0.062 5%连翘精油-0.031 25%无花果精油-0.125%丁香精油-1%紫苏提取物（体积比1∶1∶1∶1）；植物源防腐剂抑菌机理为破坏细菌的菌体细胞形态、细胞膜渗透压、遗传物质、蛋白质等结构，改变细胞膜的通透性。
Abstract:Four out of nine plant extracts were screened for their antibacterial ability and broad-spectrum against Escherichia coli，Staphylococcus aureus，and Candida albicans.The extracts were then compounded by checkerboard dilution method to obtain a plant-derived preservative，and finally，E.coli was used as the test bacteria to study its antibacterial mechanism.The results showed that the four plant extracts that were effective were：Forsythia suspensa essential oil，Ficus carica Linn essential oil，Syringa aromaticum essential oil，Perilla frutescens extract.The best formulation of the plant-derived preservative obtained by the checkerboard dilution method was 0.062 5% forsythia essential oil-0.031 25% fig essential oil-0.125% clove essential oil-1% perilla extract （volume ratio 1∶1∶1∶1）.The antibacterial mechanism of plant-derived preservatives destroyed the morphology of bacterial cells，cell membrane osmotic pressure，genetic material，protein and other structures，and changed the permeability of cell membranes.
文章编号：202201020 中图分类号： 文献标志码：
基金项目:校企合作项目（2010400023HX）
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