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食品研究与开发:2021,42(17):44-49
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沙棘叶不同溶剂提取物活性物质含量及抗氧化性研究
(1.忻州师范学院,山西 忻州 034000;2.广州万物生健康产业有限公司,广东 广州 510642;3.中国科学院烟台海岸带研究所,山东 烟台 264003)
Study on the Active Substances and Antioxidant Activity of Different Solvent Extracts of Seabuckthorn Leaves
(1.Xinzhou Teachers University,Xinzhou 034000,Shanxi,China;2.Ones Sun Health Industry Limited Company,Guangzhou 510642,Guangdong,China;3.Yantai Institute of Coastal Zone Research,Chinese Academy of Science,Yantai 264003,Shandong,China)
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投稿时间:2020-10-28    
中文摘要: 分别以甲醇、水、无水乙醇、丙酮、乙酸乙酯为提取剂,研究沙棘叶不同提取物的活性物质含量变化及抗氧化活性。结果表明:所有活性物质含量以甲醇作为提取剂,料液比为1∶10(g/mL)时含量最高,得到的总黄酮、总多糖、总有机酸、抗坏血酸、氨基酸含量分别为(19.84±0.10)、(64.26±1.38)、(3.41±10.08)、(95.55±0.26)、(7.41±0.07)mg/g。5 种提取物均具有一定的抗氧化活性,且都是在料液比1∶10(g/mL)时达到最大值,以甲醇作为提取剂,羟基自由基清除率达到最大值85.64%,以丙酮为提取剂,超氧阴离子自由基清除率和还原力达到最大值,分别为98.28%、0.85。进一步对活性物质和抗氧化活性的相关性进行分析,发现沙棘叶不同溶剂提取物活性物质与抗氧化活性呈显著正相关。
Abstract:This study aimed to investigate the antioxidant activities and active components of various solvent extracts (methanol,water,anhydrous ethanol,acetone,and ethyl acetate)of seabuckthorn leaves.The highest levels of total flavonoids,total polysaccharides,total organic acids,ascorbic acid,and amino acids,with values of(19.84±0.10),(64.26±1.38),(3.41±10.08),(95.55±0.26),and(7.41±0.07)mg/g,respectively,were observed in the methanol extract of the leaves,at a material:liquid ratio of 1∶10 (g/mL).The five solvent extracts showed relatively strong antioxidant activities at a material:liquid ratio of 1∶10(g/mL).The methanol extract showed the strongest hydroxyl radical scavenging activity of 85.64%.The acetone extract showed the strongest superoxide anion radical scavenging activity and reducing power,at 98.28% and 0.85,respectively.Further correlation analysis revealed a significant positive correlation between the content of active components and antioxidant activity in the different solvent extracts of seabuckthorn leaves.
文章编号:202117008     中图分类号:    文献标志码:
基金项目:国家自然科学基金(31700413);2019年度山西省高等学校科技创新计划项目(2019L0829);忻州市科技计划项目(20190104-2)
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