Abstract:Male Kunming mice were randomly divided into four groups of equivalent average body weight:a vehicle control group，a positive control group，and low and high dose whey peptide compound powder groups.After 30 days，each group was subjected to the forced swimming test[mice swim for 45 minute at（25±1）℃].After 35 days，prior to and after swim testing，lactate dehydrogenase（LDH），blood lactic acid（LAC），blood urea nitrogen（BUN）and hepatic glycogen content were determined.Swimming times of mice in the whey peptide compound powder groups were significantly prolonged compared with those in the control group （P＜0.01）.Mice in the whey peptide compound powder groups displayed obviously increased LDH activity and hepatic glycogen storage（P＜0.01）.Mice in the whey peptide compound powder groups displayed significantly blood lactic acid and BUN clearance after swimming（P＜0.01）.The above results indicated that whey peptide compound powder confered anti-fatigue effects.In addition，an anti-fatigue sports supplement composed of whey peptides，β-hydroxy-β-methylbutyrate（HMB），L-carnitine，and B vitamins etc.，was developed，and systematic research has been carried out，and the quality of the products was evaluated.