Abstract:The luffa leaves extract was prepared by the juice extraction-spray drying method，and a quality controllable luffa leaves extract gel was prepared with Carbomer 940 as the main matrix and then the biological activity was investigated.The total flavonoids were used as the main active quality index for quality research，and the inhibitory effect of the gel on Staphylococcus aureus，Bacillus subtilis，Escherichia coli，ABTS+free radical and DPPH free radical scavenging ability，and tyrosinase inhibitory activity were determined.The results show that the quality of the luffa leaves extract gel is controllable and stable；the total flavonoid content in the luffa leaf extract gel was （0.57±0.02）%；the antibacterial effect of Staphylococcus aureus was better；ABTS+free radicals and DPPH free radicals could be efficiently scavenged，and the antioxidant capacity was good；the gel had a tyrosinase inhibition rate of 45.09% at a concentration of 2 mg/mL，which was close to the positive control arbutin，and the tyrosinase inhibitory activity was obvious.The preparation process of the loofah leaves gel was simple，the quality was stable and controllable，and had certain external application value.