Abstract:To explore the effects of a high-fat diet（HFD）and a high-fat，high-cholesterol diet（HFHCD）on glucose and lipid metabolism，intestinal flora，and metabolites in mice，each of 3 groups of mice were fed a normal，high-fat，or high-fat，high-cholesterol diet.Compared with the normal diet group，the high-fat diet group displayed increased fasting blood glucose，impaired glucose tolerance，increased liver triglycerides，and decreased levels of propionic and butyric acids （p＜0.05）.Deoxycholic acid and cholic acid levels were significantly increased in mice fed the high fat，high cholesterol diet（p＜0.05）.Compared with the normal diet group and the high fat group，the α-diversity of the intestinal flora was decreased in the high fat，high cholesterol group，and the proportion of Firmicutes and Bacteroidetes was significantly increased （p＜0.05）.Principal coordinate analysis showed that the fecal microbiota of the three groups of mice differed significantly.