Abstract:The effect of Flammulina velutiper-derived vitaminpagenumber_ebook=34,pagenumber_book=24compound tablets on the serum biochemical indexes，bones，and organs of ovariectomized rats was investigated.The rat osteoporosis model was established by removing the ovaries.Healthy female sprague-dawley rats were divided into the sham-operated（Sham），ovariectomized（OVX），OVX+commercialpagenumber_ebook=34,pagenumber_book=24，and OVX+F.velutiperpagenumber_ebook=34,pagenumber_book=24compound tablet（OVX+MVHP）groups.The rats were treated every other day for 16 weeks.Afterward，their sera，tibias，femurs，and organs were collected.The serum calcium，phosphorus，alkaline phosphatase（AKP），and tartrate resistant acid phosphatase（StrACP）levels were determined.The organs were weighed，and the organ indexes were calculated.Additionally，bone and uterine tissue sections were prepared and stained with hematoxylin and eosin，and tissue structures were observed using a pathological image analysis system.Compared with that in the OVX group，the serum calcium concentration in the OVX+MVHP group was significantly higher（p<0.05），while the AKP concentration was significantly lower（p<0.05）.In contrast，there was no significant difference in the serum phosphorus and StrACP concentrations（p>0.05）.The heart，liver，kidney and uterus serum biochemical indexes were significantly different（p<0.05），while the spleen serum biochemical index was no（tp>0.05）.Additionally，the uterus tube and the lining of the uterus were significantly thicker（p<0.05），and there was a significant reduction in the thickness of the uterus （p<0.05）.The femur and tibia were dense，and the beam spacing as well as the bone marrow cavity were smaller.Altogether，these results indicated that F.velutiperderived pagenumber_ebook=35,pagenumber_book=25compound tablets might prevent the occurrence of postmenopausal osteoporosis.