Abstract:The essential oils from Mentha haplocalyx Briq.，Mentha asiatica Boriss.，Mentha spicata Linn.，Mentha pulegium Linn.were obtained by hydrodistillation.The essential oils were analyzed by gas chromatography-quadrupole time of flight-mass spectrometry and quantified by gas chromatography-flame ionization detector combined with internal standard method.At the same time，the 50% inhibitory concentration （IC50）of four species of Mentha L.plants essential oil to DPPH radical and ABTS+radical was determined，and the antibacterial activity of four species of Mentha L.plants essential oil was evaluated by microdilution method.The results showed that the main component of essential oils of Mentha haplocalyx Briq.was menthol（70.51%），Mentha pulegium Linn.was distinguished by high amounts of pulegone（69.30%），while the major compound in Mentha asiatica Boriss.was piperitone oxide（62.57%）.Carvone（74.00%）was the main constituent of Mentha spicata Linn..The variation among the four species of Mentha L.was linked to that of their essential oil characteristic composition.The essential oil of four different varieties of Mentha L.plants had good antioxidant activity，and had good antibacterial activity against Candida albicans and Staphylococcus aureus.