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投稿时间:2020-06-08
投稿时间:2020-06-08
中文摘要: 该文开发一种基于结构转换适配体(aptamers)的新型高灵敏度荧光“开启”适配体传感器,用于快速、灵敏检测动物源性食品中卡那霉素(kanamycin,KAN)。适配体的结构转换诱导氮掺杂石墨烯量子点(nitrogen-doped graphene quantum dots,N-GQDs)的聚集/解聚行为,从而引起体系荧光的淬灭/恢复。与之前文献方法相比,该研究方法在效率、灵敏度、选择性和稳定性等方面均表现出优异性能:线性范围为0.1 ng/mL~10.0 ng/mL,检测限低至0.036 ng/mL(S/N=3),远远低于动物源性食品中KAN的最大残留限量。并且整个检测过程(包括样品提取)可在45 min内完成。此外,该方法成功用于5种动物源性食品样品(牛奶、蜂蜜、鱼、蛋、鸡肉)中KAN的检测。
Abstract:Herein,a novel fluorescent‘turn-on’aptasensor based on a structure-switching aptamer was developed for rapid and sensitive detection of kanamycin(KAN)in animal-derived food.Its structure-switching could induce the aggregation/disaggregation behavior of nitrogen-doped graphene quantum dots(N-GQDs),which causes fluorescence quenching/recovery in the system.Compared with previous methods,this novel strategy exhibited superior behavior in terms of efficiency,sensitivity,selectivity,and stability in KAN determination:a linear range from 0.1 ng/mL to 10.0 ng/mL and a satisfactory detection limit as low as 0.036 ng/mL(S/N=3).In addition,the entire method,including sample extraction,could be completed within 45 min.Furthermore,the proposed method was applied to five animal-derived food samples(milk,honey,fish,eggs,and chicken muscle),which demonstrated practical applicability.
keywords: graphene quantum dots complementary strand fluorescent aptasensor kanamycin structures witching
文章编号:202108022 中图分类号: 文献标志码:
基金项目:
作者 | 单位 |
张磊,邓健康,张富源 | 天津科技大学食品科学与工程学院食品营养与安全国家重点实验室,天津 300457;河南科技学院食品学院,河南 新乡 453003;衡水学院生命科学学院,河北 衡水 053000;河北农业大学食品科技学院,河北 保定 071001 |
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