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食品研究与开发:2021,42(8):119-126
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基于荧光共振能量转移快速检测食品中的四环素类药物
(1.广东药科大学食品科学学院,广东 中山 528458;2.广东药科大学公共卫生学院,广东 广州 510006;3.华南农业大学兽医学院,广东 广州 510642;4.华南农业大学材料与能源学院,广东 广州 510642)
Rapid Detection of Residues of Tetracyclines in Food Based on Fluorescence Resonance Energy Transfer Immunoassay
(1.College of Food Science,Guangdong Pharmaceutical University,Zhongshan 528458,Guangdong,China;2.College of Public Health,Guangdong Pharmaceutical University,Guangzhou 510006,Guangdong,China;3.College of Veterinary Medicine,South China Agricultural University,Guangzhou 510642,Guangdong,China;4.College of Materials and Energy,South China Agricultural University,Guangzhou 510642,Guangdong,China)
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投稿时间:2020-07-16    
中文摘要: 基于上转换发光纳米技术构建荧光共振能量转移体系,快速检测食品中四环素类药物。通过将上转换发光纳米材料作为能量供体,金纳米粒子作为能量受体,建立基于荧光共振能量转移的四环素类药物的检测方法。结果表明,通过荧光量的恢复量来定量游离抗原,得到体系中荧光的恢复量与四环素类抗原浓度(范围在1 ng/mL~100 ng/mL)呈良好的线性关系,可以检测到四环素类药物的最低检出限为0.1 ng/mL。同时,该方法可用于牛奶中四环素药物的检测。通过建立能够检测四环素类药物的上转换发光纳米技术,为四环素类药物食品安全检测提供了一种快速、灵敏、稳定的方法,也为上转换发光纳米技术运用于检测食品中更多的有害物质提供了良好的基础。
Abstract:Develop fluorescence resonance energy transfer immunoassay based on upconversion fluorescence nanotechnology for the detection of tetracyclines(TCs).A method about the detection of tetracyclines based on fluorescence resonance energy transfer was established that takes the upconversion nanoparticles as an energy donor and gold nanoparticles as an energy acceptor.The free antigen was quantified by the alteration of fluorescence recovery which developed a good linear relationship between the recovery of fluorescence and the concentration of tetracycline antigen(the range was 1 ng/mL to 100 ng/mL),and the minimum detection of tetracycline was 0.1 ng/mL,it also could be used for the detection of tetracyclines in milk.An up-conversion fluorescence nanotechnology capable of detecting tetracyclines had been constructed,providing a rapid,sensitive,and stable method for food safety detection of tetracycline,it also provided a good foundation for upconversion fluorescence nanotechnology to detect more harmful substances in foods.
文章编号:202108020     中图分类号:    文献标志码:
基金项目:国家自然科学基金(31401596);广东省科技计划项目(2014A040401087、2016A020210132);广州市珠江科技新星专项资助项目(201710010003)
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