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投稿时间:2020-03-03
投稿时间:2020-03-03
中文摘要: 为探究原料对盐渍泡菜中细菌类群产生的影响,采用MiSeq高通量测序技术对两类盐渍泡菜的细菌类群进行比较分析,并采用PICRUSt对其基因功能进行预测。结果显示,发酵沙葱的香农指数、超1指数、Firmicutes(硬壁菌门)、Lactobacillus(乳酸杆菌属)、Pantoea(泛菌属)、Lentibacillus(慢生芽胞杆菌属)和 Rahnella(拉恩氏菌属)的相对含量均显著偏高(P<0.05),而菌群能量生产和转换、氨基酸转运与代谢、核苷转运与代谢和转录等功能基因的表达均显著偏低(P<0.05)。分别有68个和2个分类操作单元仅存在于所有发酵沙葱或青椒样品中,累计平均含量分别为8.40%和0.037%。经多元方差分析发现,两类盐渍泡菜细菌类群和基因功能的整体差异均显著(P<0.05)。由此可见,原料对盐渍泡菜的细菌类群与菌群基因功能的表达具有显著的影响。
Abstract:In order to explore the effect of raw materials on bacterial flora in saline pickles,MiSeq highthroughput sequencing was used to study the community structure of bacterial in 2 types saline pickles,and gene functions were predicted by PICRUSt.The results indicated that fermented Allium mongolicum Regel with higher Shannon index,Chao 1 index,and the relative abundances of Firmicutes,Lactobacillus,Pantoea,Lentibacillus and Rahnella (P<0.05),meanwhile,with lower expression of functional genes in energy production and conversion,amino acid transport and metabolism,nucleotide transport and metabolism,and transcription (P<0.05).There were 68 and 2 OTUs occurred only in all fermented Allium mongolicum Regel or Capsicum annuum var.grossum samples,with the relative abundances of 8.40% and 0.037%,respectively.The result of multivariate analysis of variance indicated that both the structure of bacterial flora and gene function were significant differences (P<0.05).Thus,materials had significant impact on the bacterial flora and gene function in saline pickles.
文章编号:202102031 中图分类号: 文献标志码:
基金项目:四川省科技厅应用基础项目(2019yj0598)
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