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食品研究与开发:2021,42(2):191-196
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原料对盐渍泡菜细菌类群及基因功能的影响
(1.湖北文理学院 食品科学技术学院 鄂西北传统发酵食品研究所,湖北 襄阳 441053;2.四川省农业科学院农产品加工研究所,四川 成都 610066)
Effect of Raw Materials on Bacterial Flora and Gene Function in Saline Pickles
(1.Northwest Hubei Research Institute of Traditional Fermented Food,College of Food Science and Technology,Hu Bei University of Arts and Science,Xiangyang 441053,Hubei,China;2.Institute of Agroproducts Processing,Sichuan Academy of Agricultural Sciences,Chengdu 610066,Sichuan,China)
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投稿时间:2020-03-03    
中文摘要: 为探究原料对盐渍泡菜中细菌类群产生的影响,采用MiSeq高通量测序技术对两类盐渍泡菜的细菌类群进行比较分析,并采用PICRUSt对其基因功能进行预测。结果显示,发酵沙葱的香农指数、超1指数、Firmicutes(硬壁菌门)、Lactobacillus(乳酸杆菌属)、Pantoea(泛菌属)、Lentibacillus(慢生芽胞杆菌属)和 Rahnella(拉恩氏菌属)的相对含量均显著偏高(P<0.05),而菌群能量生产和转换、氨基酸转运与代谢、核苷转运与代谢和转录等功能基因的表达均显著偏低(P<0.05)。分别有68个和2个分类操作单元仅存在于所有发酵沙葱或青椒样品中,累计平均含量分别为8.40%和0.037%。经多元方差分析发现,两类盐渍泡菜细菌类群和基因功能的整体差异均显著(P<0.05)。由此可见,原料对盐渍泡菜的细菌类群与菌群基因功能的表达具有显著的影响。
Abstract:In order to explore the effect of raw materials on bacterial flora in saline pickles,MiSeq highthroughput sequencing was used to study the community structure of bacterial in 2 types saline pickles,and gene functions were predicted by PICRUSt.The results indicated that fermented Allium mongolicum Regel with higher Shannon index,Chao 1 index,and the relative abundances of Firmicutes,Lactobacillus,Pantoea,Lentibacillus and Rahnella (P<0.05),meanwhile,with lower expression of functional genes in energy production and conversion,amino acid transport and metabolism,nucleotide transport and metabolism,and transcription (P<0.05).There were 68 and 2 OTUs occurred only in all fermented Allium mongolicum Regel or Capsicum annuum var.grossum samples,with the relative abundances of 8.40% and 0.037%,respectively.The result of multivariate analysis of variance indicated that both the structure of bacterial flora and gene function were significant differences (P<0.05).Thus,materials had significant impact on the bacterial flora and gene function in saline pickles.
文章编号:202102031     中图分类号:    文献标志码:
基金项目:四川省科技厅应用基础项目(2019yj0598)
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