Abstract:Due to the needs of high efficiency of food safety supervision and management could not be met by the traditional national standard detection methods of Salmonella，especially for non-prepackaged foods which are more prone to spoilage.A rapid，accurate，simple and efficient Salmonella polymerase chain reaction（PCR）molecular chromatography detection method was established for non-prepackaged foods.The national standard method and PCR molecular chromatograph are used to detect the different degrees of Salmonella contamination in 60 batches of common substrate foods（pasta products，dairy products，and meat products）and 40 batches of complex substrate foods （cold dishes and fresh eggs）.The results showed that the detection sensitivity of PCR molecular chromatography was higher for common substrate foods.And the minimal detectable concentration of positive bacteria could reach 1 CFU/mL-10 CFU/mL.The complex substrate such as fresh food and eggs were less sensitive to both detection methods.Therefore，MgCl·6H2O was added to optimize the enrichment microbial solution buffered peptone water（BPW）of Salmonella to improve the sensitivity by using the principle of increasing the osmotic pressure of enrichment solution and reducing the pH value to inhibit the growth of other interfering intestinal flora.The minimal detectable concentration of positive bacteria 104CFU/mL was reduced to 102CFU/mL and the detection sensitivity was improved by 100 times.In addition，verified by the specific experiment that PCR molecular chromatography had strong anti-interference ability.The detection of Salmonella could not be affected by other interfering strains deoxyribonucleic acid（DNA）existing in the system.The detection time of was only 22 h for the PCR molecular chromatography，which was greatly shorter than that（6 d）of the national standard method.The detection efficiency was increased by 6 times.