Abstract:In order to compare the quality of Pueraria thomsonii Benth.and Pueraria lobata（Willd.）Ohwi from different origins of Guangxi，the contents of moisture，total polysaccharides，and total isoflavones，puerarin and daidzein had been measured and compared by oven drying method，UV spectrophotometer，high performance liquid chromatography，respectively.The results showed that the contents of moisture of Pueraria thomsonii and Pueraria lobata from different origins of Guangxi were lower than 14%，the results were in line with the requirement of Chinese Pharmacopoeia 2015 version.The moisture content of Pueraria thomsonii from Guilin was 7.13% as the lowest；the contents of total polysaccharides and total isoflavones of Pueraria lobata from Yulin were the highest，which were 1.106 0 g/100 g and 5.163 0 g/100 g respectively；the contents of puerarin and daidzein of Pueraria lobata from Yulin were 41.767 5 mg/g and 5.788 4 mg/g at a higher level.The content of isoflavones in Pueraria thomsonii from Guangxi was lower than that of Pueraria lobata.The quality of Pueraria lobata from Yulin was the best after comprehensive examination of the contents of moisture，polysaccharides and total isoflavones.