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食品研究与开发:2020,41(20):137-145
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BSA-葡聚糖-叶黄素纳米颗粒的制备及其抗氧化活性
(1.天津天狮学院食品工程学院,天津 301700;2.天津商业大学生物技术与食品科学学院,天津市食品生物技术重点实验室,天津 300134;3.天津商业大学国有资产与实验室管理处,天津300134;4.江南大学食品学院,江苏无锡214122)
Preparation of BSA-Dextran-Lutein Nanoparticle and Its Anti-oxidative Properties
(1.School of Food Engineering,Tianjin Tianshi College,Tianjin 301700,China;2.School of Biotechnology and Food Science,Tianjin Key Laboratory of Food and Biotechnology,Tianjin University of Commerce,Tianjin 300134,China;3.State-owned Assets and Laboratory Management Department,Tianjin University of Commerce,Tianjin 300134,China;4.School of Food Science and Technology,Jiangnan University,Wuxi 214122,Jiangsu,China)
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投稿时间:2020-07-25    
中文摘要: 采用高压均质法制备牛血清白蛋白-叶黄素(bovine serum albumin-lutein,BSA-lutein)和牛血清白蛋白-葡聚糖-叶黄素(dextran-bovine serum albumin-lutein,DBSA-lutein)两种纳米颗粒,并对比了两者的体外抗氧化性能。研究结果表明:DBSA-lutein纳米颗粒的包封率、平均粒径、聚合物分散性指数以及Zeta电位分别为(95.86±0.83)%、(176.8±6.5)nm、0.176±0.07、(-30.5±0.6)mV;体外抗氧化性能(清除 DPPH 自由基、清除 ABTS+自由基、清除羟基自由基、总还原力以及细胞内抗氧化能力)方面,DBSA-lutein均高于BSA-lutein。
Abstract:BSA-lutein and BSA-dextran-lutein(DBSA-lutein)nanoparticles were prepared by high-pressure homogenization,and their antioxidant properties were compared in vitro.Results showed that the encapsulation efficiency,average particle size,polymer dispersion index and zeta potential of DBSA-lutein nanoparticle were(95.86±0.83)%,(176.8±6.5)nm,0.176±0.07 and(-30.5±0.6)mV respectively;the antioxidant activities in vitro (such as removing capacity of DPPH radicals,ABTS+radicals,hydroxyl radicals,total reducing power and intracellular antioxidant capacity)of DBSA-lutein nanoparticle were higher than those of BSA-lutein.
文章编号:202020023     中图分类号:    文献标志码:
基金项目:天津市应用基础与前沿技术研究计划项目(19JCTPJC54600);天津天狮学院自然科学研究项目(K17003);天津市重点研发计划项目(19YFLHSN00080);国家自然科学基金(31522044)
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