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食品研究与开发:2020,41(20):130-136
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超声辅助酶法提取绿芦笋可溶性膳食纤维工艺条件优化
(河南省农业科学院农副产品加工研究中心,河南郑州450002)
Optimization of Ultrasonic Assisted Enzymatic Extraction of Soluble Dietary Fiber from Green Asparagus
(Agricultural Products Processing Center,Henan Academy of Agricultural Sciences,Zhengzhou 450002,Henan,China)
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投稿时间:2019-09-23    
中文摘要: 以新鲜绿芦笋为原料,采用超声-酶法协同提取芦笋中可溶性膳食纤维,探讨纤维素酶添加量、超声时间、pH值和超声功率对可溶性膳食纤维得率的影响。以可溶性膳食纤维得率为响应值,通过Box-Behnken试验设计进行超声-酶法协同提取芦笋中可溶性膳食纤维的工艺优化。结果表明:影响芦笋可溶性膳食纤维含量的主次因素依次为酶添加量>pH值>超声时间>超声功率。最佳提取工艺为纤维素酶添加量0.065%、超声时间114 min,pH 5.50,超声功率180 W。在此条件下,提取芦笋可溶性膳食纤维含量得率最高,验证试验得到的得率为8.807 mg/g。
Abstract:The fresh green asparagus were used as raw materials.Ultrasonic-enzymatic method was used to extract the water-soluble dietary fiber from the green asparagus.The effects of cellulase addition,ultrasonic time,pH and ultrasonic power on the yield of water-soluble dietary fiber were investigated.Based on the yield of water-soluble dietary fiber,the Box-Behnken design was used to optimize the process of ultrasonic-enzymatic extraction of water-soluble dietary fiber from discarded asparagus.The results showed that the main factors affecting the content of water-soluble dietary fiber in asparagus were enzyme addition,pH,ultrasonic time and ultrasonic power.The optimal extraction process was cellulase addition 0.065%,ultrasonic time 114 min,pH 5.5,and ultrasonic power 180 W.Under these conditions,the extraction yield of asparagus water-soluble dietary fiber was the highest,and the yield obtained from the verification experiment was 8.807 mg/g.
文章编号:202020022     中图分类号:    文献标志码:
基金项目:河南省科技攻关项目(182102110026)
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