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食品研究与开发:2020,41(20):145-150
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超声辅助提取蓝靛果果实多糖的优化及清除自由基活性研究
(东北农业大学文理学院,黑龙江哈尔滨150030)
Optimization of Ultrasonic-assisted Extraction of Polysaccharide from Blue Honeysuckle and Scavenging Radicals Activity
(College of Art and Science,Northeast Agricultural University,Harbin 150030,Heilongjiang,China)
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投稿时间:2019-11-18    
中文摘要: 该文在单因素试验的基础上,利用响应面法优化超声波辅助蓝靛果多糖提取工艺。统计分析结果表明影响蓝靛果多糖提取率大小因素依次为:提取时间>超声功率>液料比。最佳提取条件为:时间41 min,液料比为41∶1(mL/g),超声功率310 W。在此条件下,多糖得率为(8.31±0.23)%。清除自由基试验结果表明,蓝靛果多糖对DPPH·、O2-·均有一定的清除作用,最大清除率分别为(53.92±0.88)%和(67.79±1.01)%,半抑制率浓度IC50分别为5.40 mg/mL和0.28 mg/mL。
Abstract:Based on the single factor experiment,the response surface method was used to optimize the extraction process of ultrasonic-assisted extraction polysaccharides from blue honeysuckle (Lonicera caerulea L.)fruits.The statistical analysis indicated that order of effects on the yield of blue honeysuckle polysaccharides:time>ultrasonic power>liquid-to-liquid ratio.The optimal conditions were:extraction time 41 min,liquid-to-liquid ratio 41 ∶1 (mL/g),and ultrasonic power 310 W.Under these conditions,the actual measured yield of polysaccharide was(8.31 ± 0.23)%.The polysaccharide of blue honeysuckle had a certain scavenging effects on DPPH·and O2-·.The scavenging rates on DPPH·and O2-·were (53.92 ± 0.88)% and (67.79 ± 1.01)%,respectively,and IC50values were 5.40 mg/mL and 0.28 mg/mL,individually.
文章编号:202020024     中图分类号:    文献标志码:
基金项目:黑龙江省大学生创新创业训练计划项目(201910224081)
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