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投稿时间：2019-11-08
投稿时间：2019-11-08
中文摘要: 采用超声波辅助乙醇溶剂萃取法提取香蕉中的5-羟色胺，提取收率以埃尔利希试剂分光光度法测定。通过单因素试验，考察乙醇的体积分数、超声时间、超声温度以及料液比4个因素对香蕉中5-羟色胺提取收率的影响，在此基础上，利用Box-Behnken原理设计响应面法优化5-羟色胺的提取工艺条件。结果表明，5-羟色胺最佳提取工艺条件为：料液比1∶20（g/mL），乙醇体积分数72%，超声时间33 min、超声温度32℃。此工艺条件下香蕉中5-羟色胺提取收率为32.986 7 mg/g，预测值和实际测定值接近。
Abstract:The 5-hydroxytryptamine （5-HT）in banana was extracted by ultrasonic assisted ethanol solvent extraction，and the extraction yield was determined by Ehrlich reagent spectrophotometry.The effects of four factors including ethanol volume fraction，ultrasonic time，ultrasonic temperature and solid-liquid ratio on the extraction yield of 5-HT in banana were investigated by single factor experiment.On this basis，response surface methodology was designed to optimize the extraction process conditions of 5-HT using the Box-Behnken principle.The results showed that the optimal extraction conditions of 5-HT were as follows：1 ∶20（g/mL）solid-liquid ratio，ethanol volume fraction of 72%，ultrasonic time of 33 min，and ultrasonic temperature of 32℃.Under this process condition，the extraction yield of 5-HT in banana was 32.986 7 mg/g，and the predicted value was close to the actual measured value.
keywords: banana 5-hydroxytryptamine（5-HT） solvent ultrasound-assisted extraction response surface methodology
文章编号：202020021 中图分类号： 文献标志码：
基金项目:广东省岭南特色果蔬加工关键技术及应用工程技术研究中心项目（粤科函产学研字[2015]1487号）；广东普通高校食品科学创新团队项目（2016KCXTD020）
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