Abstract:This research regarded Spirulina platensis F-1070 as experimental subjects，exploring an effectual and convenient microalgae preservation method that was able to have acquisition timely，less space required and simple operating steps.F-1070 was enduring low temperature obtained by single factor experimental way of 10 strains of algae.At the same time，the optimal concentration range of trehalose was determined.The storage conditions of preservation method were further optimized by orthogonal test.The experiment design was based on regrowth curve of preservation microalgae fitting by SLogistic regression models.The growth rate curve of microalgae was gained by the first-order derivation of the growth curve.Through the statistical analysis of both curves，this method got some characteristic indexes to estimate the microalgae vitality and evaluate the effect of preservation of microalgae concentrate in order to obtain the best conditions of the dim light low-temperature preservation method.And the preservation conditions of the obtained microalgae were 2×106CFU/mL-4×106CFU/mL of algae liquid and 15% trehalose in low light.