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投稿时间：2019-10-10
投稿时间：2019-10-10
中文摘要: 以采自内蒙古阿拉善地区的传统发酵阿拉善双峰驼酸驼乳为材料，采用乳酸细菌培养基（MRS）对其中的乳酸菌进行初分离，经平板划线法纯化后，借助16 SrDNA的序列分析法进行分子鉴定，并对其耐酸性、产酸能力、最适pH值及耐盐性等性质进行研究。结果表明，分离纯化出3株疑似优势乳酸菌，分别为乳杆菌属的Lactobacillus plantarum strain KC28、Lactobacillus plantarum strain K25和Lactobacillus sp.D1501。3株菌均为革兰氏阳性，耐酸性较好，产酸性能适中；最适培养pH值为6.5。
Abstract:In this study the traditional fermentational shubat of Alashan bactrian camel in Inner Mongolia was used as an example，the Lactic acid bacterias were initially separated using the MRS medium，further purified by flat scribing and identified by 16 SrDNA sequence analysis.Then，their acid resistance，acid production ability，optimum pH value and halotolerance etc.were studied.The results showed that separated and purified three suspected dominant Lactic acid bacterias which divided into Lactobacillus and were Lactobacillus plantarum strain KC28，Lactobacillus plantarum strain K25 and Lactobacillus sp.D1501，respectively.All 3 strains were gram-positive that possessed good acid resistance，moderate acid production performance and halotolerance.The optimum pH value were 6.5.
文章编号：202018031 中图分类号： 文献标志码：
基金项目:国家自然科学基金项目（31560443）；内蒙古自治区科技成果转化项目（CGZ2018122）；内蒙古自然科学基金项目（2017MS0306）
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