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投稿时间：2019-09-08
投稿时间：2019-09-08
中文摘要: 以人参残渣为原料，通过单因素和响应面试验研究加酶量、料液比、粒度以及酶解时间等因素对淀粉酶以及蛋白酶提取人参渣中不可溶性膳食纤维的影响。结果显示，酶解的最佳条件为：人参渣粉碎，过80目筛，按料液比1∶25（g/mL）与蒸馏水混合，调pH值至6.0，加入0.5%的淀粉酶，60℃恒温酶解1 h；再调节pH值至7.5，加入0.5%的蛋白酶，45℃酶解1 h。在此条件下，人参渣膳食纤维提取率为68.11%。
Abstract:Using ginseng residue as raw material，the effects of enzyme dosage，ratio of material to liquid，particle size and hydrolysis time on the extraction of insoluble dietary fiber from ginseng residue were studied by single factor and response surface test.The results showed the optimum conditions of enzymatic hydrolysis were determined as follows：ginseng residue was crushed by ultra-fine crusher，passing 80 mesh screen，mixing with distilled water at 1 ∶25（g/mL）according to the ratio of material to liquid，adjusting pH to 6.0，0.5%amylase was added and hydrolyzed at 60℃for 1 h.When pH was adjusted to 7.5，0.5%protease was added and hydrolyzed at 45℃for 1h.Under these conditions，the extraction rate of dietary fiber from ginseng residue was 68.11%.
keywords: ginseng dietary fiber amylase protease response surface
文章编号：202016018 中图分类号： 文献标志码：
基金项目:吉林省科技发展计划项目（20180201046NY）
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