本文已被：浏览 1006次 下载 232次
投稿时间：2019-08-17
投稿时间：2019-08-17
中文摘要: 为研究黄绿蜜环菌硒多糖的抗氧化活性及抑菌活性，采用乙醇沉淀法、Sevag法从黄绿蜜环菌富硒培养液中获得硒多糖，体外测定其对羟基自由基、DPPH自由基和ABTS+自由基的清除能力，并采用滤纸片扩散法测定对大肠杆菌和金黄色葡萄球菌的抑制作用。经测定，黄绿蜜环菌硒多糖中硒和多糖含量分别为（2.84±0.23）g/kg和（56.27±0.35）%。研究结果表明，硒多糖对羟基自由基、DPPH自由基、ABTS+自由基的清除能力随着其浓度的升高而显著增加（P＜0.05），但清除率均小于抗坏血酸。高浓度的黄绿蜜环菌硒多糖对大肠杆菌（Escherichia coli）生长的抑制效果明显高于低浓度（P＜0.05），但对金黄色葡萄球菌（Staphylococcus aureus）无抑制作用。综上所述，黄绿蜜环菌硒多糖具有一定的抗氧化活性和抑菌效果，其生物活性强弱与浓度有关。
Abstract:In order to investigate antioxidant and antimicrobial activities of selenium-containing polysaccharide extracted from Armillaria luteo-virens，selenium-containing polysaccharide were partitioned from Se-enriched fermentation by ethanol precipitation method and Sevag method in turn，and evaluated in vitro the·OH，DPPH·and ABTS+·scavenging activity，respectively，and assessed antimicrobial activity using agar diffusion method.The results showed that the concentrations of selenium and polysaccharide in selenium-containing polysaccharide were（2.84±0.23）g/kg and（56.27±0.35）%，respectively.The significantly enhancing removal of·OH，DPPH·and ABTS+·scavenging activity in vitro were observed with the increasing selenium-containing polysaccharide（P＜0.05）.However the DPPH，hydroxyl and ABTS+radical scavenging potential of seleniumcontaining polysaccharide were lower than ascorbic acid.In addition，The high concentrations of seleniumcontaining polysaccharide inhibited significantly the growth of Escherichia coli compared to the low concentrations （P ＜0.05），without the inhibitory effect on Staphylococcus aureus.Selenium-containing polysaccharide from A.luteo-virens had certain antioxidant and antimicrobial activities.There were some correlations between activities and concentrations.
keywords: Armillaria luteo-virens selenium-containing polysaccharide antioxidant activity antimicrobial activity
文章编号：202015002 中图分类号： 文献标志码：
基金项目:国家自然科学地区科学基金项目（31760697）；青海师范大学本科生科技创新项目（qhnuxskj2019018）
引用文本：
津ICP备20000603号-1