Abstract:A rapid and accurate method for identifying bovine-derived components in beef products and quantifying the content of beef components was established.The bovine mitochondrial cytochrome b gene was used as a target gene to design specific primers.The eukaryotic ribosomal 16SrDNA was selected as an internal reference gene，and real-time fluorescence relative quantification was used.The logarithm of the percentage of beef mass had a good linear relationship with the corresponding cycle threshold difference ΔCt.The standard curve regression formula was y =-3.364 5x +0.873 7，R2=0.992 6，and the amplification efficiency reached 98.25 %.The quality of the standard curve was evaluated by simulating mixed samples，which proved that the method was suitable for the identification and content detection of beef components.It could provide reference comments for research on quantifying beef composition in meat products.