Abstract:To establish an enzyme-linked immunosorbent assay for the determination of furacillin metabolites in animal derived food.Using the furacillin metabolite antigen and antibody as the basic raw materials，indirect competitive enzyme-linked immunosorbent assays （ELISA）method was established and its performance was measured by optimizing the reaction conditions.The established detection method was linear within the range of 0.03 μg/L-2.43 μg/L.The detection sensitivity was 0.03 μg/kg and the detection limits of fish tissue samples and shrimp tissue samples were 0.236 μg/kg and 0.229 μg/kg，respectively.The recoveries were between 75%and 120 %，the variation coefficient between batch and batch were below 15 %，compared with standard method of high performance liquid chromatography-mass spectrum/mass spectrum （LC-MS/MS），two methods used in the detection of fish samples were showing good correlation，the correlation coefficient R2 of 0.994 4.The sensitivity，accuracy and precision of the established method for the determination of furacillin metabolites by enzyme-linked immunosorbent were in line with the requirements of the method for the detection of veterinary drug residues in food of animal origin in China.