Abstract:High performance liquid chromatography（HPLC）was used to establish the characteristic fingerprint of safflower produced in Yunnan.The HPLC fingerprint of safflower produced in Yunnan was established by using the computer software of “similarity evaluation system 2004A version of traditional Chinese medicine chromatography fingerprint”，and the similarity of 25 batches of safflower in Yunnan was evaluated by using Agilun 1100 UV detection HPLC.A total of 14 chromatographic peaks were obtained from 25 batches of samples collected from different producing areas，such as Dali and Lijiang，Yunnan，and their basic characteristics were the same，and the similarity of fingerprints of safflower from different producing areas in Yunnan was different.The hydroxysafflor yellow A was a flavonoid compound in the safflower，the property was stable，the peak was relatively prominent，and the peak at the middle position of the chromatographic peak could be used as a reference.The fingerprint of the high performance liquid chromatography （HPLC）be used to mark the characteristic chromatogram of the chemical constituents of the safflower，and provides a perfect information for the identification and research of the Chinese medicinal materials.The method was simple and accurate，the economy was convenient and the repeatabilitywas well，and a certain scientific basis was provided for the quality control of the safflower medicinal material.