Abstract:Taking the frozen dumplings as the research object，the microbial diversity of frozen dumplings before freezing was studied.And it could provide reference for the storage period and anti-corrosion technology of quick-frozen dumplings to avoid the health problems in the later stage.The bacteria in the dumpling meat were separated by a traditional separation and culture method to obtain 30 strains.The morphological identification，genomic DNA extraction，polymerase chain reaction （PCR） amplification，16S rDNA sequencing and sequence alignment analysis were used to identify the genus of these strains and construct the population structure evolution tree.The results showed that there were 14 genera and 22 species in 30 strains，and the dominant bacteria were distributed in the genus Pseudomonas in the γ-Proteobacteria.