Abstract:The main objective of the study was to determine the effects of ultrasound treatment on the modification of zein and inulin by glycosylation and the secondary structure content in zein-inulin were studied to investigate the improvement of mechanical properties.Zein was conjugated with inulin by glycosylation under dry-heating condition with ultrasound-assisted modification.In this study，the grafting degree，tensile strength，partical size，elongation and disulfide bond changes were determine.The ultrasonic power was 500 W，the degree of grafting（DG）was 28.65%，the tensile strength（TS）and elongation of zein-inulin films was 19.4 MPa and 3.6%，respectively.An increase of the amount of sulfhydryl group and a decrease of the amount of disulfide bond in protein structure of zein was found accompanied by an increase of the ultrasonic power.The secondary structure contents by far-UV Circular Dichroism Spectroscopy（CD）were monitored.It indicated that secondary structures of zein-inulin have changed and the α-helix reduced by 11.9% and β-pleated sheets increased by 11.3%.The results showed the structure can be changed of zein，it were improved by ultrasound treatment.