Abstract:Poria cocos was used as a fermentation strain and Salvia miltiorrhiza was used as a drug matrix to carry out two-way fermentation experiments in the basic fermentation medium.The optimal culture time of the seed liquid was determined，and the Salvia miltiorrhiza's participation ratio and fermentation time were optimized.The optimal incubation time of the seed liquid was 1 day，the optimal addition ratio of Salvia miltiorrhiza was 1 g/mL，and the optimal fermentation time was 5 days.Comparing the effects of cofermentation system and single system on the content of intracellular polysaccharides，extracellular polysaccharideand total flavonoids，the results showed that the co-fermentation system significantly increased the content of extracellular polysaccharides （15.74 mg/mL），intracellular polysaccharides （1.26 mg/mL）and total flavonoids （1.04 mg/mL）in the system compared with the single system.Comparing the effects of cofermentation system and single system on blood glucose levels in diabetic mice，the results showed that the cofermentation system could effectively reduce the blood glucose of diabetic mice and improve the glucose tolerance of diabetic mice compared with the single system.The co-fermentation product of the sputum-danshen prepared had potential hypoglycemic effect and further development value.