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投稿时间：2019-01-03
投稿时间：2019-01-03
中文摘要: 建立超声辅助低共熔溶剂结合液液萃取技术提取葡萄皮中甲霜灵、吡虫啉、多菌灵3 种农药的方法。采用ChCl-乙二醇（1 ∶2/mol ∶mol）和 ChCl-丙三醇（1 ∶4/mol ∶mol）比例为体积比 1 ∶1 混合体系，用量 2 mL，超声时间 5 min，pH 值为 5，进行提取。色谱条件，色谱柱：Venusil MP C18（250 mm×4.6 mm，5 μm）；流速：1.0 mL/min；柱温：30 ℃；检测波长：280 nm 和 210 nm；进样量：20 μL；流动相：甲醇（A）-0.05%磷酸水溶液（B）；梯度洗脱。多菌灵、吡虫啉、甲霜灵的提取回收率分别为多菌灵91%，吡虫啉112%，甲霜灵85%。3 种成分在线性范围内关系良好。超声辅助低共熔溶剂结合液液萃取方法能有效地提取出葡萄皮中甲霜灵、吡虫啉、多菌灵3 种农药。
Abstract:A kind of green ultrasound-assisted dispersive liquid-liquid microextraction method was established to extract the residues of metalaxyl，imidacloprid and carbendazim in grapeskin.The sample was extracted by a mixture of choline chloride and ethylene glycol（1 ∶2/mol ∶mol）and choline chloride and glycerol（1 ∶4/mol ∶mol）with the volume ratio of 1 ∶1 through ultrasonic for 5 min with volume of 2 mL and pH of 5.The chromatographic separation was performed on a Venusil MP C18（250 mm×4.6 mm，5 μm）at 30 ℃ with methanol （A）-0.05%phosphoric acid aqueous solution （B）as mobile phase in gradient elution mode.The wavelength of 280 nm and 210 nm were utilized.The extraction recovery rates of metalaxyl，imidacloprid and carbendazim were 91%，112%and 85%respectively.The three components showed good linear relationships and the recovery rates can meet the requirements of the method.The present experiment suggests that DESs are efficiently extract solvents in ultrasound-assisted dispersive liquid-liquid microextraction method for residual analysis of metalaxyl，imidacloprid and carbendazim in grapeskin.
keywords: deep eutectic solvent pesticide residue grapeskin ultrasound-assisted dispersive liquid-liquid microextraction response surface methodology
文章编号：202003033 中图分类号： 文献标志码：
基金项目:
| Author Name | Affiliation |
| CHEN Su-e，LIU Liang-liang，ZHAO Long-shan，ZHU Lin | Shanxi Health Vocational College，Jinzhong 030619，Shanxi，China；Shenyang Pharmaceutical University，Shenyang 110016，Liaoning，China |
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