本文已被：浏览 1486次 下载 350次
投稿时间：2019-03-06
投稿时间：2019-03-06
中文摘要: 旨在研究副干酪乳杆菌VL8 胞外多糖（Lactobacillus paracasei VL8 exopolysaccharides，VL8-EPS）对巨噬细胞RAW264.7 的免疫激活作用。试验采用不同浓度的VL8-EPS 作用于RAW264.7 细胞，设空白对照组，脂多糖（lipopolysaccharide，LPS）处理组（终浓度为 10 μg/mL）和 VL8-EPS 处理组（终浓度为 50、100、200、400、800 μg/mL）。迪夫快速染色法观察细胞形态；比色法测定诱导型一氧化氮合酶（inducible nitric oxide synthase，iNOS）活力；WST-1 法测定超氧化物歧化酶（superoxide dismutase，SOD）活力；微量法检测超氧阴离子（O2-）的含量；钼酸显色法检测过氧化氢（H2O2）的分泌量。结果表明，VL8-EPS 能够改变细胞形态，剂量依赖性提高RAW264.7 细胞内iNOS、SOD 酶活力，促进细胞分泌O2-和H2O2。在800 μg/mL 时，较空白对照组分别提高了104.64%、10.30%、666.87%、81.78%。说明VL8-EPS 通过提高胞内酶活性及释放活性氧激活RAW264.7 细胞从而发挥免疫增强作用。
Abstract:To studied the immunological activation of exopolysaccharides from Lactobacikkus paracasei VL8（VL8-EPS）on macrophages RAW264.7.Different concentrations of VL8-EPS were applied to RAW264.7 cells，includied the control group，lipopolysaccharide（LPS）group（10 μg/mL）and VL8-EPS group（50，100，200，400，800 μg/mL）.The morphological of the cells were observed using the Diff-Quik Stain；the activity of inducible nitric oxide synthase （iNOS）was determined by colorimetry；the activity of superoxide dismutase（SOD）was determined by the WST-1 method；the content of oxygen free radical O2- was detected by micro method；the secretion of H2O2 was detected by molybdate chromogenic method.The results showed that VL8-EPS change the cell morphology，increased the activity of iNOS and SOD in RAW264.7 cells in a dosedependent manner and promote the secretion of O2-and H2O2.At 800 μg/mL，it was increased by 104.64 %，10.30 %，666.87 % and 81.78 %，respectively，compared with the blank control group.VL8-EPS activated RAW264.7 cells by increasing intracellular enzyme activity and releasing reactive oxygen species.
keywords: Lactobacillus paracasei exopolysaccharides macrophage intracellular enzyme reactive oxygen species
文章编号：202003034 中图分类号： 文献标志码：
基金项目:国家十三五重点研发计划（2017YFC1601704）；河北省科技支撑计划科研专项（16227109D）
| Author Name | Affiliation |
| LI Qi，DU Jia-feng，GAO Jie，LIU Xiao-yu，WANG Xiang-hong，SANG Ya-xin | College of Food Science and Technology，Hebei Agricultural University，Baoding 071001，Hebei，China |
引用文本：
津ICP备20000603号-1