Abstract:Ultrasonic-assisted extraction，solid phase extraction and high performance liquid chromatography by orthogonal experiment optimization were adopted to explore a simple，fast and efficient method for extraction of gibberellic acid in fruits and vegetables.Results showed that the best extraction conditions by orthogonal experiment optimization were as follows：ultrasonic time，45 min；ultrasonic power，128 W；solid to liquid ratio，1 ∶4.5 （g/mL）；methanol concentration，85 %.The yield of gibberellic acid was 285.19 mg/kg under these optimized conditions.The method was used for determination of gibberellic acid residues in ten kinds of commercially available fruits and vegetables with recovery rates ranged from 79.36 % to 97.05 % and relative standard deviations ranged from 2.63%to 9.99%.The results could meet the requirements for pesticide residue analysis，applicable to determination of gibberellic acid residues in a wide variety of fruits and vegetables.