Abstract:The accelerated solvent extraction process of total flavonoids from chestnut leaves was optimized by response surface methodology，and the antioxidant activity was investigated.The effects of cycle times，extraction temperature，extraction time and ethanol volume fraction on the extraction rate of total flavonoids from chestnut leaves were investigated by factor experiments.Box-Behnken design was performed to optimize the extraction conditions.The antioxidative capacities of chestnut leaves extracts cultured were evaluated using DPPH，ABTS+and FRAP assays.The results showed that the optimum extraction conditions were 2 cycles for 7.3 min（extrac－tion time）at 73 ℃ （extraction temperature）with 40%ethanol （volume fraction）.Under these optimal conditions，the extraction rate of flavonoids reached the maximum of （5.18±0.06）%，which was well stabilized with the predicted value of （5.20±0.10）%.At the maximum chestnut leaves flavonoid extract concentration，the DPPH free radical scavenging rate was 88.44%，the ABTS+free radical scavenging rate was 82.76%，the total antioxidant capacity of chestnut leaves flavonoids were 980.3 μmol/L，and which indicated that the antioxidant activity of chestnut leaves flavonoid was strong.