Abstract:Dahong tangerine peel Pu'er tea，produced by Chakeng Village，Xinhui District，Jiangmen City，Guangdong Province，was used as raw material.Total flavonoids were extracted from the tangerine peel and Pu'er tea with an ultrasonic-assistance.The extract was then purified by AB-8 macroporous resin，and concentrated by rotary evaporation.The total flavonoids from tangerine peel and Pu'er tea were mixed in the proportion of 3 ∶1，1 ∶1 and 1 ∶3，the scavenging capacity to·OH ，ABTS+·and DPPH free radicals and total reducing power of VC and the total flavonoids of mixture，tangerine peel and Pu'er tea were determined.The results demonstrated that the total flavonoids from both tangerine peel and Pu'er tea had a good antioxidant activity.The IC50 mix of free radical scavenging rate of each compound group was less than IC50add，and there was a statistically significant difference at the 95%confidence level between them，he interaction index（γ）was less than 1 in each group between total flavonoids from tangerine peel and Pu'er tea，which indicated a synergistic antioxidant effect.