Abstract:The fruit quality，contents of moisture，ash，crude protein，crude fat，and fatty acid in 19 kinds of walnut samples from different regions belonging to Guangxi and Yunnan were analyzed by methods of the Chinese national standard.The results showed that the range of contents of moisture，ash，crude protein，and crude fat were consistent with other previous studies and the Chinese national standard，and significant differences were found in the protein contents among all samples.The crude fat content of all samples was higher than 65%，except for Nandan Zhongbao（NDZB），and that of Fengshan Dingmao（FSDM）was the highest.An obvious significant difference was found in kernel yield，and yield of crude fat of FSDM and Yunxin（YX）walnut was the highest.The highest content of unsaturated fatty acid （UFA），monounsaturated fatty acid（MUFA），and polyunsaturated fatty acid（PUFA）was FSDM，Jinchengzi（JCZ），and YX，respectively.The top three varieties of unsaturated fatty acid content in whole walnut fruit were Yuelibae （YLBE），FSDM，and YX；polyunsaturated fatty acid was YX，NDZB，and FSDM.Regarding fatty acid ratio of ω6/ω3，Niangqing（NQ）Yangza Guangming（YZGM）and Longjia（LJ） were lower than that of the other samples.Thus，nutrient contents of 19 walnut samples from different regions of Guangxi and Yunnan were analyzed.The data obtained from fatty acid types in kernel or whole fruit，even or by ω6/ω3 plus with different fatty acid types，was beneficial to select good qualification from different walnut varieties in Guangxi and Yunnan.