Abstract:Using Lyopyllum cinerascens as raw material，the influence of liquid-solid ratio，extraction time，extraction temperature，ultrasonic power，ethanol concentration and extraction times on the extraction effect of polyphenols from Lyopyllum cinerascens was studied.On this basis，the response surface method was utilized to optimize the process conditions of ultrasonic assisted extraction of polyphenols from Lyopyllum cinerascens.With adsorption and desorption effect as evaluation index，the optimum macroporous resin was selected and the optimum purification process for polyphenols from Lyopyllum cinerascens was determined.The results showed that the optimum extraction process was as follows：liquid-solid ratio 22 ∶1（mL/g），extraction temperature 67 ℃and ethanol concentration 71%.Under these conditions，the extraction amount of polyphenol was 14.60 mg/g.The D101 resin was selected as the optimum macroporous resin for the purification of polyphenols from Lyopyllum cinerascens.The optimum purification process of the polyphenols from Lyopyllum cinerascens was as follows：sample pH 3，ethanol concentration 50%，sample flow rate 0.5 mL/min，sample concentration 0.8 mg/mL，elution rate 1 mL/min and elution dosage 4 BV.After purification，the content of the polyphenols was （71.18±0.9）mg/g，which was nearly 3.9 times higher than that of crude polyphenols，indicating that the purification effect was good.