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投稿时间：2018-10-10
投稿时间：2018-10-10
中文摘要: 采用高效液相色谱-荧光检测技术，建立蔬菜和水果中15种欧盟优控多环芳烃（European priority controlled polycyclic aromatic hydrocarbons，EU-PAHs）的同时测定方法。利用超声波辅助乙腈提取蔬菜和水果中的15种欧盟优控多环芳烃，加入N-丙基乙二胺（primary secondary amine，PSA）和无水MgSO4净化，经0.22 μm滤膜过滤，用Waters PAH色谱柱分离，柱温35℃，用乙腈-水作流动相梯度洗脱，流速为1.2 mL/min，采用荧光检测器测定15种欧盟优控多环芳烃的含量；进样体积为20 μL。按照选定的色谱条件，15种EU-PAHs在50 min内达到基线分离，方法的精密度为 4.2%～12.0%，方法回收率 83.6%～97.2%。15种 EU-PAHs的线性范围为 0.2 μg/kg～8.0 μg/kg，相关系数均大于0.999以上。
Abstract:A method for the simultaneous determination of 15 European priority controlled polycyclic aromatic hydrocarbons（EU-PAHs）in vegetables and fruits by high performance liquid chromatography with fluorescence detector was developed.Ultrasound assisted acetonitrile extraction of EU-PAHs from vegetables and fruits samples，purified by primary secondary amine（PSA）and MgSO4，filtered with 0.22 μm millipore filter.A total of 15 EU-PAHs were chromatographically separated on a PAH column，the column temperature was 35 ℃，and gradiently elutedby the mobile phase composed of acetonitrile and aqueoussolution.The flow rate was1.2 mL/min.15 European priority controlled polycyclic aromatic hydrocarbons was detected by fluorescence detector.The injection volume was 20 μL.After optimization of chromatographic conditions，15 kinds of EU-PAHs achieved baseline separation within 50 min.The precision of the method was 4.2%-12.0%，and the method recovery rate was 83.6%-97.2%.The linear range of the other 15 kinds of EU-PAHs was 0.2 μg/kg-8.0 μg/kg.The correlation coefficients were all greater than 0.999.
keywords: vegetables and fruits chromatography high performance liquid European priority controlled polycyclic aromatic hydrocarbons
文章编号：201917031 中图分类号： 文献标志码：
基金项目:
| Author Name | Affiliation |
| WANG Zhong，HE Yi，WANG Min，XING Yan，YIN Mao-rong | Zibo Center for DiseasesControl and Prevention，Zibo 255026，Shandong，China |
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