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投稿时间：2019-05-07
投稿时间：2019-05-07
中文摘要: 以党参多糖（Codonopsis pilosula polysaccharide，CPP）为研究对象，在HNO3-BaCl2催化下，利用Na2SeO3合成党参多糖硒酸酯（Codonopsis pilosula polysaccharide selenate，CPPS）。考察温度、Na2SeO3与CPP的质量比值、HNO3体积分数对合成的影响，并进一步通过响应面法优化CPPS制备的最佳工艺，同时采用紫外和红外光谱对CPPS进行结构表征。结果表明CPPS的最佳合成条件为：反应温度70℃，Na2SeO3与CPP的质量比值为1，HNO3体积分数为0.6%，制得的CPPS中硒含量为3.06 mg/g，模型拟合良好，CPPS中硒以Se=O的形式存在。抗氧化试验表明合成的CPPS对Fe2+和超氧阴离子自由基具有一定的清除能力。
Abstract:The Codonopsis pilosula polysaccharide （CPP） and sodium selenite were used to synthesize Codonopsis pilosula polysaccharide selenate （CPPS）under the catalysis of HNO3and BaCl2.The influence of temperature，the mass ratio of Na2SeO3to CPP and the volume fraction of HNO3on the synthesis of CPPS were investigated，then the optimum preparation process of CPPS was further optimized by response surface methodology，and the structure of CPPS was characterized by ultraviolet and infrared spectroscopy.The results showed that the optimum synthetic conditions of CPPS were as follows：the reaction temperature was 70℃，the mass ratio of Na2SeO3to CPP was 1 and the volume fraction of HNO3was 0.6%，finally the selenium content in CPPS was 3.06 mg/g and the model fitted well with selenium in CPPS existed in the form of Se=O Antioxidant experiments showed that the CPPS synthesized had a certain scavenging capacity for Fe2+and superoxide anions radical.
keywords: Codonopsis pilosula polysaccharide polysaccharide selenate response surface methodology structure characterization anti-oxidation
文章编号：201917017 中图分类号： 文献标志码：
基金项目:
| Author Name | Affiliation |
| SHU Chang，XIA Jie，YUAN Shuai，ZHANG Xi-feng，YAN You-yu | SchoolofBiologicalandPharmaceuticalEngineering，WuhanPolytechnic University，Wuhan430023，Hubei，China |
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