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投稿时间：2018-10-09
投稿时间：2018-10-09
中文摘要: 研究优化葛仙米多糖提取工艺，在单因素试验的基础上，以料液比、浸提时间、浸提温度为考察因素，运用响应面法优化葛仙米多糖的提取工艺。葛仙米多糖最佳工艺条件为：料液比1∶230（g/mL），浸提温度97℃、浸提时间110 min，在该条件下，葛仙米多糖的提取率为29.14%，与理论值30.64%相近。结果与模型预测值吻合度高，说明该模型切实可行，同时为葛仙米多糖的加工利用及相关研究提供了参考依据。
Abstract:For improving the extraction process of the Nostoc sphaeroides Kützing polysaccharide （NSKP）by response surface methodology，the solid-liquid，extraction temperature and extraction time were selected as the investigation factors by the single-factor experiment.The optimum extraction conditions were found to be extraction at 97℃ for 110 min with a solvent-to-solid ratio of 1∶230（g/mL）.Experiments conducted under these conditions gave a polysaccharide yield of 29.14%which was closed to the theoretical value of 30.64%.The results were in good agreement with the predicted value of the model，which indicated that the model was reliable.At the same time，this study provided a reference for processing and utilization of NSKP.
keywords: Nostoc sphaeroides Kützing polysaccharide single-factor response surface methodology extraction process
文章编号：201917016 中图分类号： 文献标志码：
基金项目:湖南省食品药品监督管理局食品药品安全科技项目（R201714）；湖南省创新创业技术投资专项项目（S2018GXJSZ0015）
| 作者 | 单位 |
| 李洪权，柏玉冰，谢文剑，刘晓红，刘飞波，张瑞华，周熙 | 株洲市食品药品检验所，湖南株洲412000；湖南中医药大学第一附属医院，湖南长沙410007；安乡县中医院，湖南常德415600；湖南省株洲炎帝生物责任有限公司，湖南株洲412000 |
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