Abstract:Microbial growth and reproduction are the main factors affecting the shelf life of chilled venison.By improving the way of pretreatment，the initial colonies that cool the venison can be effectively controlled.The pretreatment of venison was optimized by taking acetic acid concentration，ultraviolet irradiation time and distance as the influencing factors.The optimum pretreatment conditions were determined by single factor experiment and response surface experiment.The results showed that after spraying with 2%acetic acid solution，the ultraviolet irradiation was 120 s at a distance of 53.38 cm from the ultraviolet lamp.Under these conditions，the initial colony of venison was significantly reduced by 97.5%.